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mhgf elisa kit  (R&D Systems)


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    R&D Systems mhgf elisa kit
    Mhgf Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 84 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mhgf+elisa/Mouse%2FRat+HGF+Quantikine+ELISA+Kit/bio_rxiv__64898__2026__02__24__707802-248-6-9
    Average 94 stars, based on 84 article reviews
    mhgf elisa kit - by Bioz Stars, 2026-09
    94/100 stars

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    Enzyme-linked Immunosorbent Assay:

    Article Title: Hepatocyte Growth Factor Regulates Macrophage Transition to the M2 Phenotype and Promotes Murine Skeletal Muscle Regeneration
    Article Snippet: TAs were prepared and mechanistically homogenized using Bullet Blender Storm (Next Advance, NY), and total proteins were extracted in RIPA lysis buffer (Sigma Aldrich, MO) containing a protease inhibitor (Roche, Basel, Switzerland), phosphatase inhibitor (Roche, Basel, Switzerland), and PMSF (Sigma Aldrich, MO). .. Samples were centrifuged at 12,000 rpm for 15 min at 4°C, and the supernatants containing total protein were subjected to mHGF ELISA (R&D systems, MN) following the manufacturer’s protocol. .. For immunoblotting, TAs or cultured cells were prepared and homogenized in RIPA lysis buffer (Sigma Aldrich, MO) containing a protease inhibitor (Roche, Basel, Switzerland) and phosphatase inhibitor (Roche, Basel, Switzerland) using Bullet Blender Storm (Next Advance, NY).



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    mhgf duoset  (R&D Systems)
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    Prrx1b–Hgf signaling regulates pancreatic cancer cell invasion. ( A ) Prrx1a and Prrx1b target genes identified by ChIP-seq. Genes of enriched pathways (KEGG) are indicated. ( B ) Relative <t>mHgf</t> gene expression in control and Prrx1a-overexpressing and Prrx1b-overexpressing KP fl CY cells ( left ) and control and Prrx1a and Prrx1b knockdown KPC2 cells ( right ). (*) P < 0.05; mean ± standard deviation (SD). ( C ) Invasion assay in control and Prrx1a and Prrx1b knockdown cells using mrHGF. ( D ) Double IHC staining for PRRX1B (brown) and mHGF (blue) in KP fl CY pancreatic tumors (orthotopic transplantation model). Bars, 50 μm. ( E ) Quantification of secreted mHGF protein by ELISA. n = 3; mean ± SEM. ( F ) Secreted mHGF protein in the supernatant of control and Prrx1a/b knockdown KPC2 cells. n = 5; mean ± SEM.
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    Prrx1b–Hgf signaling regulates pancreatic cancer cell invasion. ( A ) Prrx1a and Prrx1b target genes identified by ChIP-seq. Genes of enriched pathways (KEGG) are indicated. ( B ) Relative mHgf gene expression in control and Prrx1a-overexpressing and Prrx1b-overexpressing KP fl CY cells ( left ) and control and Prrx1a and Prrx1b knockdown KPC2 cells ( right ). (*) P < 0.05; mean ± standard deviation (SD). ( C ) Invasion assay in control and Prrx1a and Prrx1b knockdown cells using mrHGF. ( D ) Double IHC staining for PRRX1B (brown) and mHGF (blue) in KP fl CY pancreatic tumors (orthotopic transplantation model). Bars, 50 μm. ( E ) Quantification of secreted mHGF protein by ELISA. n = 3; mean ± SEM. ( F ) Secreted mHGF protein in the supernatant of control and Prrx1a/b knockdown KPC2 cells. n = 5; mean ± SEM.

    Journal: Genes & Development

    Article Title: Prrx1 isoform switching regulates pancreatic cancer invasion and metastatic colonization

    doi: 10.1101/gad.263327.115

    Figure Lengend Snippet: Prrx1b–Hgf signaling regulates pancreatic cancer cell invasion. ( A ) Prrx1a and Prrx1b target genes identified by ChIP-seq. Genes of enriched pathways (KEGG) are indicated. ( B ) Relative mHgf gene expression in control and Prrx1a-overexpressing and Prrx1b-overexpressing KP fl CY cells ( left ) and control and Prrx1a and Prrx1b knockdown KPC2 cells ( right ). (*) P < 0.05; mean ± standard deviation (SD). ( C ) Invasion assay in control and Prrx1a and Prrx1b knockdown cells using mrHGF. ( D ) Double IHC staining for PRRX1B (brown) and mHGF (blue) in KP fl CY pancreatic tumors (orthotopic transplantation model). Bars, 50 μm. ( E ) Quantification of secreted mHGF protein by ELISA. n = 3; mean ± SEM. ( F ) Secreted mHGF protein in the supernatant of control and Prrx1a/b knockdown KPC2 cells. n = 5; mean ± SEM.

    Article Snippet: mHGF-secreted protein levels were measured in the supernatant of cultured cells as well as mouse serum using the mHGF DuoSet (R&D Systems, DY2207) according to the manufacturer's protocol.

    Techniques: ChIP-sequencing, Gene Expression, Control, Knockdown, Standard Deviation, Invasion Assay, Immunohistochemistry, Transplantation Assay, Enzyme-linked Immunosorbent Assay